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I can't recall what issue it was (I think it was Volume 1, Number 12), but in it there was a panel of one of the turtles (Raphael, I think) getting out of the shower and complaining that Donatello didn't fix the water heater yet in April's old farmhouse. So, with that in mind, yes they take showers.
And they make sure to wash their tails so they can go to the bathroom without risk of infection.
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