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Well there is that one episode later on where Shredder shoots a ray gun at Napoleon which causes him to transform into a stronger frog.
But yeah, they never did fully explain what long-term affects of the mutagen were in the old toon. It was obviously permanent, but do they change again later on? Splinter was mutated for years though and never changed.
I always gathered the gamera Raph in Vol. 4 and mutated Don in the 2k3 series Season 4 were pretty much the same thing. (I know the cause is different, vampires and Bishop's formula, but you get what I mean).
It had an effect on their mutations.
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